how to make normalization for your data in graphpad prism 8

81901257 (to YXW) the Natural Science Foundation of Jiangsu Province of China, No. KYCX20_2818 (to WL) and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, to Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education), Conflict of Interest: None BK20180951 (to YXW) Postgraduate Research and Practice Innovation Program of Jiangsu Province of China, No. There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.

In this study, we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes: Actb, Ankrd27, CypA, Gapdh, Hprt1, Mrpl10, Pgk1, Rictor, Rn18s, Tbp, Ubc, Ubxn11, and Ywhaz. Our results show that the expression of Ankrd27, Ubc, and Tbp were stable after spinal cord injury, while Actb was the most unstable internal control gene.

How to make normalization for your data in graphpad prism 8